英文全名
Recombinant Human Noggin (rHuNoggin)
英文别名
蛋白结构
氨基酸序列
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
Accession
Gene ID
产品英文概述
来源
分子量
Approximately 46.3 kDa, non-disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg in the presence of 5 ng/ml rHuBMP-4.
外观
Sterile Filtered White lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
内毒素
Less than 1 EU/ug of rHuNoggin as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
1. Davis SWandCamper SA. 2007. Dev Biol, 305: 145-60.2. Zhu W, Kim J, Cheng C, et al. 2006. Bone, 39: 61-71.3. Oxley CD, Rashid R, Goudie DR, et al. 2008. Horm Res, 69: 221-6.4. Bayramov AV, Eroshkin FM, Martynova NY, et al. 2011. Development, 138: 5345-56.5. Secondini C, Wetterwald A, Schwaninger R, et al. 2011. PLoS One, 6: e16078.
纯度
> 95 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
5ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Noggin是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Noggin (rHuNoggin)
