英文全名
Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10)
英文别名
蛋白结构
氨基酸序列
LGQDMVSPEA TNSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS
Accession
Gene ID
产品英文概述
来源
分子量
Approximately 19.1 kDa, a single, non-glycosylated polypeptide chain containing 169 amino acids.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
外观
Sterile Filtered White lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4.
内毒素
Less than 1 EU/ug of rHuKGF-2/FGF-10 as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.5. Entesarian M, Matsson H, Klar J, et al. 2005. Nat Genet. 37:125-7.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
5ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Keratinocyte Growth Factor-2/FGF-10是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Keratinocyte Growth Factor-2/FGF-10 (rHuKGF-2/FGF-10)
