英文全名
Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4)
英文别名
HST, HST-1, HSTF-1, HBGF-4, Transforming Protein KS3
蛋白结构
氨基酸序列
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL
Accession
Gene ID
产品英文概述
FGF-4, also named FGFK and KFGF, belongs to the fibroblast growth factor (FGF) family. By signaling through the FGF R1c, 2c, 3c and 4 receptors, FGF-4 has functions that maintain a population of progenitor cells in the epiblast that generates mesoderm, and contribute to the stem cell population that is incorporated in the tailbud. It is also required for axial elongation of the mouse embryo after gastrulation. Mature human FGF4 (71206 a.a.) shares 91 %, 82 %, 94 % and 91 % a.a. identity with murine, rat, canine and bovine FGF4. Additionally, FGF-4 shares about 30 % sequence identity with the prototypical members of the FGF family.
来源
Escherichia coli.
分子量
Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4, 300 mM NaCl.
内毒素
Less than 1 EU/ug of rHuFGF-4 as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 500 mM NaCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
纯度
> 96 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
5ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Fibroblast Growth Factor 4是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Fibroblast Growth Factor 4 (rHuFGF-4)
