英文全名
Recombinant Human Ciliary Neurotrophic Factor (rHuCNTF)
英文别名
CNTF
蛋白结构
氨基酸序列
MAFTEHSPLT PHRRDLCSRS IWLARKIRSD LTALTESYVK HQGLNKNINL DSADGMPVAS TDQWSELTEA ERLQENLQAY RTFHVLLARL LEDQQVHFTP TEGDFHQAIH TLLLQVAAFA YQIEELMILL EYKIPRNEAD GMPINVGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRFIS SHQTGIPARG SHYIANNKKM
Accession
Gene ID
产品英文概述
来源
Escherichia coli.
分子量
Approximately 22.9 kDa, a single non-glycosylated polypeptide chain containing 200 amino acids.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 150 ng/ml, corresponding to a specific activity of > 6.7 × 103 IU/mg.
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
内毒素
Less than 1 EU/ug of rHuCNTF as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
1. Lam A, Fuller F, Miller J, et al. 1991. Gene. 102:271-6.2. Bazan JF. 1991. Neuron. 7:197-208.3. McGregor NE, Poulton IJ, Walker EC, et al. 2010. Calcif Tissue Int. 86:261-70.4. Lambert PD, Anderson KD, Sleeman MW, et al. 2001. Proc Natl Acad Sci U S A. 98:4652-7.
纯度
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
5ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Human Ciliary Neurotrophic Factor是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Ciliary Neurotrophic Factor (rHuCNTF)
