英文全名

Recombinant Human Tumor Necrosis Factor-beta/TNFSF1 (rHuTNF-beta/TNFSF1)

英文别名

蛋白结构

氨基酸序列

Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 5 pg/ml, corresponding to a specific activity of > 2.0 × 108 IU/mg in the presence of actinomycin D.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH7.4, with 0.02 % Tween-20.

内毒素

Less than 0.1 EU/ug of rHuTNF-beta/TNFSF1 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 96 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human Tumor Necrosis Factor-beta/TNFSF1是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Tumor Necrosis Factor-beta/TNFSF1 (rHuTNF-beta/TNFSF1)

英文全名

Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade)

英文别名

LR3 IGF-I

蛋白结构

氨基酸序列

MFPAMPLSSL FVNGPRTLCG AELVDALQFV CGDRGFYFNK PTGYGSSSRR APQTGIVDEC CFRSCDLRRL EMYCAPLKPA KSA

Accession

Gene ID

产品英文概述

IGF-1 belonged to the insulin gene family, is a mitogenic polypeptide growth factor that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. It is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. The LR3IGF-1 is a long-term analog of human IGF-1, specifically designed and manufactured for mammalian cell culture to support large-scale manufacturing of recombinant biopharmaceuticals.

来源

Escherichia coli.

分子量

Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.

生物活性

Assay 1: Fully biologically active when compared to standard. Measured in a serum-free cell proliferation assay using human MCF-7 cells. The ED50 for this effect is 0.3-1.5 ng/ml, corresponding to a specific activity of > 6.7 × 105 U/mg.
Assay 2: Fully biologically active when compared to standard. The ED50 as determined by the stimulation of protein synthesis using rat L6 myoblasts is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 U/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.2.

内毒素

Less than 0.01 EU/ug of rHuLR3 IGF-1 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.-Refer to lot specific COA for the Use by Date when stored at 2 to 8 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Conlon MA, Tomas FM, Owens PC, et al. 1995. J Endocrinol, 146: 247-53.2. Dunaiski V, Dunshea FR, Walton PE, et al. 1997. J Endocrinol, 155: 559-65.3. Garnaut SM, Howarth GS, Read LC. 2002. Growth Factors, 20: 17-25.4. Xi G, Kamanga-Sollo E, Pampusch MS, et al. 2004. J Cell Physiol, 200: 387-94.5. Hadsell DL, Parlow AF, Torres D, et al. 2008. J Endocrinol, 198: 61-70.

纯度

> 98 % by SDS-PAGE analyses.>90 % by RP-HPLC analyses.

SDS-PAGE电泳

包装规格

1mg/5mg/20mg

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human LR3 Insulin-like Growth factor-1, Media Grade (rHuLR3IGF-1,MediaGrade)

英文全名

Recombinant PreScission Protease (rPPase)

英文别名

3C protease, Picornain 3C, PSP

蛋白结构

氨基酸序列

Accession

Gene ID

产品英文概述

PreScission protease is a cysteine protease derived from human rhinovirus – HRV3C Protease. rPP is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. It specifically recognizes the amino acid sequences which include the core site of Leu-Phe-Gln-Gly-Pro and cleaves between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the super structures of the fusion protein. rPP works most effective at 4°C and can digest substrates at room temperature as well.

来源

Escherichia coli.

分子量

PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease. The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues. Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.

生物活性

外观

Sterile colorless liquid.

配方

50 mM Tris-HCl, pH 7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use.

内毒素

溶解说明

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

参考资料

1. Werner G, Rosenwirth B, Bauer E, et al. 1986. J Virol, 57: 1084-93.2. Libby RT, Cosman D, Cooney MK, et al. 1988. Biochemistry, 27: 6262-8.3. Aschauer B, Werner G, McCray J, et al. 1991. Virology, 184: 587-94.4. Leong LE, Walker PA, Porter AG. 1993. J Biol Chem, 268: 25735-9.

纯度

One unit is defined as the amount of enzyme needed to cleave 100 ug of fusion protein in 16 hours to 90 % completion at 5 °C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

SDS-PAGE电泳

包装规格

100IU/250IU

安全数据表下载 (SDS)

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技术参数表下载 (TDS)

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品牌链接

Recombinant PreScission Protease是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant PreScission Protease (rPPase)

英文全名

Recombinant Streptavidin (rStreptavidin)

英文别名

蛋白结构

氨基酸序列

MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS

Accession

P22629

Gene ID

产品英文概述

来源

分子量

~52,000 per tetramer

生物活性

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized in 10 mM potassium phosphate buffer pH 6.5.

内毒素

Less than 0.1 EU/ug of rStreptavidin as determined by LAL method.

溶解说明

Dissolve with double distilled water

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- Refer to lot specific COA for the Use by Date when stored at ≤ -20 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses

SDS-PAGE电泳

包装规格

1mg/25mg/100mg

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

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品牌链接

Recombinant Streptavidin是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Streptavidin (rStreptavidin)

英文全名

Recombinant Human Insulin-like Growth factor-2 (rHuIGF-2)

英文别名

蛋白结构

氨基酸序列

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE

Accession

E3UN46

Gene ID

3481

产品英文概述

来源

分子量

Approximately 7.5 kDa, a single non-glycosylated polypeptide chain containing 67 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

外观

Sterile Filtered White lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 150 mM NaCl, with 0.02 % Tween-20.

内毒素

Less than 1 EU/ug of rHuIGF-2 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

点击下载

技术参数表下载 (TDS)

点击下载

品牌链接

Recombinant Human Insulin-like Growth factor-2是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Insulin-like Growth factor-2 (rHuIGF-2)