ChemWhat重组蛋白归档 - 第56页共57页 - Watson(沃森)

ChemWhat重组蛋白, 产品

Recombinant Human Cyclophilin D (rHuCyP-D)

英文全名

Recombinant Human Cyclophilin D (rHuCyP-D)

英文别名

PPIF, Cyclophilin F, CyP-M, Rotamase F

蛋白结构

氨基酸序列

CSKGSGDPSS SSSSGNPLVY LDVDANGKPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FTLKHVGPGV LSMANAGPNT NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGRTSKKIV ITDCGQLS

Accession

Gene ID

产品英文概述

CyP-D is a part of the peptidyl-prolyl cis-trans isomerase (PPIase) family. CyP-D accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. CyP-D is key component of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.

来源

Escherichia coli.

分子量

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

生物活性

No data available

外观

Sterile colorless liquid.

配方

Supplied as a 0.2 um filtered solution in PBS, pH7.4, with 1 mM DTT, 10 % glycerol.

内毒素

Less than 0.1 EU/ug of rHuCyP-D as determined by LAL method.

溶解说明

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 6 months from date of receipt, -20 to -70 °C as supplied.- 3 months, -20 to -70 °C under sterile conditions after opening.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/500ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Cyclophilin D是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Cyclophilin D (rHuCyP-D)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Interleukin-10 GMP (rHuIL-10)

英文全名

Recombinant Human Interleukin-10 GMP (rHuIL-10)

英文别名

CSIF

蛋白结构

氨基酸序列

SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN

Accession

Gene ID

产品英文概述

Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.

来源

Escherichia coli.

分子量

Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 U/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 6.0, with 0.02 % Tween-20.

内毒素

Less than 0.01 EU/ug of rHuIL-10 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Eskdale J, Kube D, Tesch H, et al. 1997. Immunogenetics. 46:120-8.2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11.3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Interleukin-10 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interleukin-10 GMP (rHuIL-10)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Murine Neuropoietin (rMuNeuropoietin)

英文全名

Recombinant Murine Neuropoietin (rMuNeuropoietin)

英文别名

Ctf2

蛋白结构

氨基酸序列

APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA

Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 200 ng/ml, corresponding to a specific activity of > 5000 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 500 mM NaCl, with 0.5 mM DTT.

内毒素

Less than 1 EU/ug of rMuNeuropoietin as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug/500ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Murine Neuropoietin是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Murine Neuropoietin (rMuNeuropoietin)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Interleukin-12 (rHuIL-12)

英文全名

Recombinant Human Interleukin-12 (rHuIL-12)

英文别名

Natural killer cell stimulatory factor chain 2, NKSF2, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40

蛋白结构

Squence of Recombinant Human Interleukin-12

氨基酸序列

HuIL-12 p40: Ile23 – Ser328; Accession # P29460HuIL-12 p35: Arg23 – Ser219; Accession # P29459

Accession

Gene ID

产品英文概述

IL-12 is a heterodimeric cytokine of disulfide-linked p35 and p40 subunits. It is secreted by monocytes, macrophages, dendritic cells, neutrophils, Langerhans cells, keratinocytes, microglia, and peripheral B cells. In humans, the p40 subunit can also associate with a p19 subunit to form IL-23. IL-12 signals through a receptor complex consisting of IL-12 R beta 1 and IL-12 R beta 2. It promotes Th1 immune responses by inducing IFN-gamma secretion from NK cells, T cells, and macrophages and by enhancing NK cell and T cell mediated cytotoxicity. It also cooperates with IL-18 in the generation of Th1 memory cells.

来源

Spodoptera frugiperda, Sf21 (baculovirus)

分子量

Apparent molecular mass of 60 kDa in SDS-PAGE under non-reducing conditions, 41 kDa and 29 kDa under reducing conditions, a disulfide-linked heterodimer consisting of the 306 amino acid rHuIL-12 p40 and the 197 amino acid rHuIL-12 p35 subunits.

生物活性

Measured in a cell proliferation assay using PHA-stimulated human T lymphoblasts. The ED50 for this effect is 0.01-0.05 ng/mL. The specific activity of rHuIL-12 is approximately 1.1 × 104 units/ug, which is calibrated against rHuIL-12 WHO Standard (NIBSC code: 95/544).

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.2.

内毒素

Less than 1.0 EU/ug of rHuIL-12 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriately buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 97 % by SDS-PAGE.

SDS-PAGE电泳

SDS-PAGE of Recombinant Human Interleukin-12

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Interleukin-12是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interleukin-12 (rHuIL-12)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Rhesus Macaque Interleukin-5 (rRhIL-5)

英文全名

Recombinant Rhesus Macaque Interleukin-5 (rRhIL-5)

英文别名

Eosinophil differentiation factor, TRF

蛋白结构

氨基酸序列

IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES

Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

Approximately 26.1 kDa, a disulfide-linked homodimer of two 115 amino acid polypeptide chains.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.

内毒素

Less than 0.1 EU/ug of rRhIL-5 as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/100ug/500ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Rhesus Macaque Interleukin-5是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Rhesus Macaque Interleukin-5 (rRhIL-5)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Interferon-gamma GMP (rHuIFN-gamma)

英文全名

Recombinant Human Interferon-gamma GMP (rHuIFN-gamma)

英文别名

Immune Interferon, type II interferon, T cell interferon, MAF

蛋白结构

氨基酸序列

MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRGR RASQ

Accession

Gene ID

产品英文概述

Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. Human IFN-gamma is highly species specific and is biologically active only in human and primate cells. IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. IFN-gamma also augments TH1 cell expansion and may be required for TH1 cell differentiation.

来源

Escherichia coli.

分子量

Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 144 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as measured in anti-viral assays using human HeLa cells infected with encephalomyocarditis (EMC) virus is 0.15-0.80 ng/ml.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 5.0, with 3 % Trehalose.

内毒素

Less than 0.01 EU/ug of rHuIFN-gamma GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Pennino D, Bhavsar PK, Effner R, et al. 2012. J Allergy Clin Immunol, 2. Hibi M, Hachimura S, Ise W, et al. 2003. Cytotechnology, 43: 49-55.3. Wang H, Ruan Z, Wang Y, et al. 2008. Mol Immunol, 45: 1548-56.4. Kopinski P, Przybylski G, Jarzemska A, et al. 2007. Pol Merkur Lekarski, 23: 15-21.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Interferon-gamma GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Interferon-gamma GMP (rHuIFN-gamma)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human NT-pro-BNP (rHuNT-pro-BNP)

英文全名

Recombinant Human NT-pro-BNP (rHuNT-pro-BNP)

英文别名

Natriuretic peptides B, Gamma-brain natriuretic peptide

蛋白结构

氨基酸序列

HPLGSPGSAS DLETSGLQEQ RNHLQGKLSE LQVEQTSLEP LQESPRPTGV WKSREVATEG IRGHRKMVLY TLRAPR

Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

Approximately 8.5 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.

生物活性

No data available

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in 20mM Tris-HCl, pH 8.0, 150mM NaCl.

内毒素

Less than 0.1 EU/ug of rHuNT-pro-BNP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.-Refer to lot specific COA for the Use by Date when stored at ≤ -20 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

100ug/500ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human NT-pro-BNP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human NT-pro-BNP (rHuNT-pro-BNP)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Epidermal Growth Factor GMP (rHuEGF)

英文全名

Recombinant Human Epidermal Growth Factor GMP (rHuEGF)

英文别名

Urogastrone

蛋白结构

氨基酸序列

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Accession

Gene ID

产品英文概述

Epidermal Growth Factor (EGF) was originally discovered in crude preparations of nerve growth factor prepared from mouse submaxillary glands as an activity that induced early eyelid opening, incisor eruption, hair growth inhibition, and stunting of growth when injected into newborn mice. Human EGF was isolated from urine based on its inhibitory effect on gastric secretion and named urogastrone, accordingly. EGF is prototypic of a family of growth factors that are derived from membrane-anchored precursors. All members of this family are characterized by the presence of at least one EGF structural unit (defined by the presence of a conserved 6 cysteine motif that forms three disulfide bonds) in their extracellular domain. EGF is initially synthesized as a 130 kDa precursor transmembrane protein containing 9 EGF units. The mature soluble EGF sequence corresponds to the EGF unit located proximal to the transmembrane domain. The membrane EGF precursor is capable of binding to the EGF receptor and was reported to be biologically active. Mature human EGF shares 70 % a.a. sequence identity with mature mouse and rat EGF.

来源

Escherichia coli.

分子量

Approximately 6.2 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 0.01 EU/ug of rHuEGF GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Chevalier RL, Goyal S, Thornhill BA. 1999. J Urol, 162: 1532-6.2. Gehm BD, McAndrews JM, Jordan VC, et al. 2000. Mol Cell Endocrinol, 159: 53-62.3. Yang H, Sun X, Wang Z, et al. 2003. J Membr Biol, 194: 47-58.4. Cohen S. 2008. J Biol Chem, 283: 33793-7.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Epidermal Growth Factor GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Epidermal Growth Factor GMP (rHuEGF)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Enhanced Green Fluorecence Protein (reGFP)

英文全名

Recombinant Enhanced Green Fluorecence Protein (reGFP)

英文别名

eGFP

蛋白结构

氨基酸序列

MVSKGEELFT GVVPILVELD GDVNGHKFSV SGEGEGDATY GKLTLKFICT TGKLPVPWPT LVTTLTYGVQ CFSRYPDHMK QHDFFKSAMP EGYVQERTIF FKDDGNYKTR AEVKFEGDTL VNRIELKGID FKEDGNILGH KLEYNYNSHN VYIMADKQKN GIKVNFKIRH NIEDGSVQLA DHYQQNTPIG DGPVLLPDNH YLSTQSALSK DPNEKRDHMV LLEFVTAAGI TLGMDELYK

Accession

Gene ID

产品英文概述

来源

Escherichia coli.

分子量

Approximately 26.9 kDa, a single non-glycosylated polypeptide chain containing 239 amino acids.

生物活性

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.

内毒素

Less than 1 EU/ug of reGFP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

纯度

> 95 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

10ug/500ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Enhanced Green Fluorecence Protein是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Enhanced Green Fluorecence Protein (reGFP)

2021年12月23日/通过： great_watson-int

ChemWhat重组蛋白, 产品

Recombinant Human Insulin-like Growth factor-1 GMP (rHuIGF-1)

英文全名

Recombinant Human Insulin-like Growth factor-1 GMP (rHuIGF-1)

英文别名

Somatomedin C, IGF-I, IGF-IA

蛋白结构

氨基酸序列

GPETLCGAEL VDALQFVCGD RGFYFNKPTG YGSSSRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPAKSA

Accession

Gene ID

产品英文概述

The insulin-like growth factors (IGFs) belonged to the insulin gene family, are mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. The IGFs are similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. The production of IGF-1 is stimulated by growth hormone (GH) and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5B. Recombinant human IGF-1 are globular proteins containing 70 amino acids and 3 intra-molecular disulfide bonds. Mature human IGF-1 shares 94 % and 96 % a.a. sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity.

来源

Escherichia coli.

分子量

Approximately 7.6 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids.

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

外观

Sterile filtered white lyophilized (freeze-dried) powder.

配方

Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.0.

内毒素

Less than 0.01 EU/ug of rHuIGF-1 GMP as determined by LAL method.

溶解说明

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.A minimum of 12 months when stored at ≤ -20 °C as supplied. Refer to lot specific COA for the Use by Date.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.

参考资料

1. Skottner A, Fryklund L, Hansson HA. 1986. Acta Paediatr Scand Suppl, 325: 107-11.2. Bartlett WP, Li XS, Williams M. 1992. Brain Res Mol Brain Res, 12: 285-91.3. Palmade F, Sechoy-Chambon O, Coquelet C, et al. 1994. Curr Eye Res, 13: 531-7.4. Tennagels N, Hube-Magg C, Wirth A, et al. 1999. Biochem Biophys Res Commun, 260: 724-8.5. Laron Z. 2004. Novartis Found Symp, 262: 56-77; discussion -83, 265-8.6. Shiratsuchi I, Akagi Y, Kawahara A, et al. 2011. Anticancer Res, 31: 2541-5.

纯度

> 98 % by SDS-PAGE and HPLC analyses.

SDS-PAGE电泳

包装规格

5ug/100ug

安全数据表下载 (SDS)

技术参数表下载 (TDS)

品牌链接

Recombinant Human Insulin-like Growth factor-1 GMP是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Human Insulin-like Growth factor-1 GMP (rHuIGF-1)

2021年12月23日/通过： great_watson-int