英文全名
Recombinant Rat Interleukin-13, 113a.a. (rRtIL-13,113a.a.)
英文别名
T-cell Activation Protein P600
蛋白结构
氨基酸序列
TPGPVRRSTS PPVALRELIE ELSNITQDQK TSLCNSSMVW SVDLTAGGFC AALESLTNIS SCNAIHRTQR ILNGLCNQKA SDVASSPPDT KIEVAQFISK LLNYSKQLFR YGH
Accession
Gene ID
产品英文概述
Interleukin-13 (IL-13) is expressed by the IL13 gene and secreted by many cell types, especially T helper type 2 (Th2) cells. The high solution from of IL-13 reported to be a monomer with two internal disulfide bonds that contribute to a bundled four alpha-helix configuration. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Mature rat IL-13 shares 59 %, 75 %, and 60 % amino acid sequence identity with human, mouse, and rhesus IL-13, respectively.
来源
Escherichia coli.
分子量
Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 113 amino acids.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in 2 × PBS, pH 7.4, 5 % trehalose.
内毒素
Less than 1 EU/ug of rRtIL-13, 113a.a. as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
纯度
> 98 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
10ug/100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Rat Interleukin-13, 113a.a.是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Rat Interleukin-13, 113a.a. (rRtIL-13,113a.a.)
