英文全名
Recombinant Rat Interferon-gamma (rRtIFN-gamma)
英文别名
蛋白结构
氨基酸序列
QGTLIESLES LKNYFNSSSM DAMEGKSLLL DIWRNWQKDG NTKILESQII SFYLRLFEVL KDNQAISNNI SVIESHLITN FFSNSKAKKD AFMSIAKFEV NNPQIQHKAV NELIRVIHQL SPESSLRKRK RSRC
Accession
Gene ID
产品英文概述
Interferon-gamma (IFN-gamma), also known as Type II interferon or immune interferon, is a cytokine produced primarily by T-lymphocytes and natural killer cells. The protein shares no significant homology with IFN-β or the various IFN-alpha family proteins. Mature IFN-gamma exists as noncovalently-linked homodimers. It shares high sequence indentity with mouse IFN-gamma (86 %). IFN-gamma was originally characterized based on its antiviral activities. The protein also exerts antiproliferative, immunoregulatory and proinflammatory activities and is thus important in host defense mechanisms. IFN-gamma induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-gamma augments TH1 cell expansion and may be required for TH1 cell differentiation.
来源
Escherichia coli.
分子量
Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acids.
生物活性
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using murine L929 cells infected with encephalomyocarditis (EMC) virus is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
外观
Sterile filtered white lyophilized (freeze-dried) powder.
配方
Lyophilized from a 0.2 um filtered concentrated solution in 1 × PBS, pH 7.4, 1 mM DTT, 5 % Trehalose and 0.05 % Tween-80.
内毒素
Less than 0.1 EU/ug of rRtIFN-gamma as determined by LAL method.
溶解说明
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
参考资料
纯度
> 97 % by SDS-PAGE and HPLC analyses.
SDS-PAGE电泳
包装规格
100ug/500ug
安全数据表下载 (SDS)
技术参数表下载 (TDS)
品牌链接
Recombinant Rat Interferon-gamma是ChemWhat品牌授权产品并通过沃尔森销售，下面是ChemWhat上的该产品链接 Recombinant Rat Interferon-gamma (rRtIFN-gamma)
